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RESEARCH ARTICLE
BIOSCIENCE RESEARCH, 2026
23(1):12-18.
OPEN ACCESS
Genetic authentication of medically significant legumes of Fabaceae family
using the standardized DNA barcoding method
Sakina1*, Ghazala
H Rizwani1,2, Syed Rizwan Ali3,4, Hakim Abdul Bari4,
Muhammad Jahanzeb4, Sheraz Muhammad Siddiqui5,
Muhammad Amer Nazir6, Muhammad Waleed7, Muhammad
Ikhlaq5, Elaf Shaikh3 and Syeda Alishba3
1Department
of Pharmacognosy, Faculty of Pharmacy and Pharmaceutical sciences,
University of Karachi, Pakistan
2Hafiz Muhammad Ilyas Institute
of Pharmacology &Herbal Sciences, Hamdard University, Pakistan
3Human
Nutrition & dietetics, Faculty of Eastern Medicine, Hamdard University,
Pakistan
4Hamdard
Laboratories Waqf Pakistan, Karachi-75270, Pakistan
5Faculty
of Eastern Medicine, Hamdard University, Pakistan
6Department
of Applied Sciences, Faculty of Engineering Sciences, Hamdard University,
Pakistan
7Department
of Horticulture, Hamdard University, Pakistan
DOI: https://doi.org/10.65970/br.2024.51
Abstract
Growing global demand for herbal
medicine and nutritional supplements has increased due to the high usage of
traditional herbs in recent decades. Various plant materials are in high
demand as a result of the growing popularity of herbal goods. Chemical
approaches, macroscopic and microscopic investigation, and plant taxonomy
are examples of traditional identification techniques. Global commercial
interest in traditional herbs identification at genetic level has increased
due to high risk of adulteration in the herbs. The main method used to
identify herbs genetically is DNA barcoding. The ability to determine the
material's purity is the main advantage of this method. In this study, four
medically significant plants were purchased from the local herbal market in
Karachi. Using polymerase chain reaction technology and sequencing of the
universal internal transcribed spacer region (ITS2) a nuclear
barcode region, samples were accurately identified. The selected bean
samples were identified as Plant A: Vigna mungo; Plant B: Lens
culinaris; Plant C: Clitoriaternateas; and Plant D:
Vignaradiata, appears to have a ribosomal nuclear ITS2 region
that exhibits more than 90% similarity with the reference barcode sequences
indicating a high rate of species identification and discrimination.
Therefore, amplification and sequencing a universal barcode region in the
DNA offers a revolutionary tool for identifying species, preserving genetic
diversity, and using plant species responsibly that have medical importance.
Keywords: DNA barcoding, internal transcribed spacer region,
adulteration, Polymerase chain reaction, Amplification
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