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RESEARCH ARTICLE                      BIOSCIENCE RESEARCH, 2026 23(1): 19-24.                     OPEN ACCESS

Comparative Evaluation of Chia (Salvia hispanica L.) Plants Grown under in Vitro Culture and Natural Terrain Conditions

Yeşim Karaand Nesrin Erim

Pamukkale University, Department of Biology, Faculty of Science, Denizli, Türkiye

DOI: https://doi.org/10.65970/br.2026.136

Abstract

Chia seeds were grown under different environmental conditions: Greenhouse Conditions (GC) and Natural Terrain Conditions (NTC). The physiological characteristics of the plants were determined by content analysis of M1 generation seeds obtained by the plant tissue culture method, including mineral matter, oil, antioxidant content, total phenolic content, and phytochemical profile. Statistical analyses revealed a significant difference in germination rate and length of the seeds. In vitro culture of cells, tissues, organs, and components defined as sterile by others under controlled environmental conditions (temperature, humidity, and lighting). The seeds were germinated in vitro in Murashige and Skoog basal medium (MS) containing 3% sucrose, 7% agar, 0.4 mg l^-1 gibberellic acid (GA₃), and 10 mg l^-1 ascorbic acid (AA). 16 hours of light and 8 hours of darkness. In the rooting experiment, different concentrations of IBA (0, 1, 2) mg L^-1 were used, and measurements were taken after one month.

Keywords: In Vitro Culture, Natural Terrain Conditions, Chia (Salvia hispanica L.)